Name :
Human RELT Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
RELT (receptor expressed in lymphoid tissues) is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF19L). RELT is primarily expressed in hematopoietic tissues with highest levels in spleen, lymph node, thymus, peripheral blood leukocytes, bone marrow and fetal liver. It is also found in in leukemias and lymphomas. RELT has a 137 amino acid (aa) extracellular domain containing one TNFR cysteinerich repeat and a 247 aa cytoplasmic region that lacks a death domain. The ECD of RELT is most closely related to that of TROY (TNFRSF19) and OX40 (TNFRSF4). RELT has been shown to selectively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1). Overexpression of RELT can activate the NF-kB, p38 and JNK signaling pathways and induces apoptosis. RELT is also capable of stimulating T-cell proliferation in the presence of CD3 signaling. However RELT does not bind any of the TNF superfamily ligands. Two related transmembrane proteins, RELL1 and RELL2, have been identified. Both RELL1 and RELL2 can interact with RELT and may modulate its signaling activity.
Gene Symbol :
TRLT; TNFRSF19L
NCBI Gene ID :
84957
Uniprot Entry :
Q969Z4
Construct Details :
The recombinant human RELT-Fc fusion is expressed as a 365 amino acid protein consisting of Ser26 – Gln162 region of RELT (UniProt accession #Q969Z4) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human RELT-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 STTLWQCPPG EEPDLDPGQG TLCRPCPPGT FSAAWGSSPC QPHARCSLWR RLEAQVGMAT 70 80 90 100 110 120 RDTLCGDCWP GWFGPWGVPR VPCQPCSWAP LGTHGCDEWG RRARRGVEVA AGASSGGETR 130 140 150 160 170 180 QPGNGTRAGG PEETAAQSTG THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 190 200 210 220 230 240 VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK 250 260 270 280 290 300 VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE 310 320 330 340 350 360 SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK
M.W. :
Calculated molecular mass (kDa): 40; Estimated by SDS-PAGE under reducing condition (kDa): 45-55
Calculated PI :
6.74
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 80410
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Not Available
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TRLT; TNFRSF19L
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
References :
1. Blood. 97: 2702 (2001). 2. Proc.Natl.Acad.Sci. 99: 16899 (2003) 3. J. Biol. Chem. 281:13964(2006). 4. Biochem. Biophys. Res. Commun. 340:535 (2006). 5. Cell. Immunol. 261:1 (2010).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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