Name :
Human TLR1 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
TLR1 is a single-pass type I transmembrane glycoprotein belonging to the Toll-like receptor (TLR) family. The TLR family members play a fundamental role in pathogen recognition and activation of innate immunity. They serve as pattern recognition receptors for microbial pathogens. There are at least 11 mouse and 10 human TLR members. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs contain a large number of leucinerich repeats (LRRs) and a cytoplasmic tail with a Toll/IL1 receptor (TIR) domain. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR1 contains 19 LRR repeats in the extracellular region. The various TLRs exhibit different patterns of expression. TLR1 is ubiquitously expressed, and at higher levels than other TLRs. It is found on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligandindependent association with TLR2. TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols.
Gene Symbol :
TLR1; TIL; CD281; rsc786; TIL. LPRS5; KIAA0012
NCBI Gene ID :
7096
Uniprot Entry :
Q15399
Construct Details :
The recombinant human TLR1-Fc fusion protein is expressed as a 778 amino acid protein consisting of Ser25 Glu574 region of TLR1 (UniProt accession #Q15399) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image above).
Source :
Human cells stably expressing human TLR1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 SEFLVDRSKN GLIHVPKDLS QKTTILNISQ NYISELWTSD ILSLSKLRIL IISHNRIQYL 70 80 90 100 110 120 DISVFKFNQE LEYLDLSHNK LVKISCHPTV NLKHLDLSFN AFDALPICKE FGNMSQLKFL 130 140 150 160 170 180 GLSTTHLEKS SVLPIAHLNI SKVLLVLGET YGEKEDPEGL QDFNTESLHI VFPTNKEFHF 190 200 210 220 230 240 ILDVSVKTVA NLELSNIKCV LEDNKCSYFL SILAKLQTNP KLSNLTLNNI ETTWNSFIRI 250 260 270 280 290 300 LQLVWHTTVW YFSISNVKLQ GQLDFRDFDY SGTSLKALSI HQVVSDVFGF PQSYIYEIFS 310 320 330 340 350 360 NMNIKNFTVS GTRMVHMLCP SKISPFLHLD FSNNLLTDTV FENCGHLTEL ETLILQMNQL 370 380 390 400 410 420 KELSKIAEMT TQMKSLQQLD ISQNSVSYDE KKGDCSWTKS LLSLNMSSNI LTDTIFRCLP 430 440 450 460 470 480 PRIKVLDLHS NKIKSIPKQV VKLEALQELN VAFNSLTDLP GCGSFSSLSV LIIDHNSVSH 490 500 510 520 530 540 PSADFFQSCQ KMRSIKAGDN PFQCTCELGE FVKNIDQVSS EVLEGWPDSY KCDYPESYRG 550 560 570 580 590 600 TLLKDFHMSE STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE 610 620 630 640 650 660 DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP 670 680 690 700 710 720 APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN 730 740 750 760 770 NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass (kDa): 88.1; Estimated by SDS-PAGE under reducing condition (kDa): 90-100
Calculated PI :
6.16
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 88905
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to the triacylated peptide biotinylated Pam3CysSerLys4 (PAM3CSK4).
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TLR1; TIL; CD281; rsc786; TIL. LPRS5; KIAA0012
Research Area :
Immunology
Pathway/Disease :
Innate Immunity
Species :
Human
CD Antigen :
CD281
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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