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Name :
Human VNN2/Vanin-2/GPI-80 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Description :
VNN2 (vascular non-inflammatory molecule 2), also known as glycosyl- phosphatidyl inositol-anchored protein GPI-80, Vanin-2, and FOAP-4, is a GPI-anchored cell membrane protein belonging to the Vanin/BTD/VNN family of the carbon-nitrogen (CN) hydrolase family. The Vanin family includes secreted and membrane- associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. VNN2 shows high sequence similarity with other Vanin family members, and also with biotinidase. No biotinidase activity has been demonstrated for any of the vanin proteins. VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. The pantetheinase activity of VNN2 may play a role in oxidative-stress response. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is involved in the thymus homing of bone marrow cells. It may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil. Elevated VNN2 expression is associated with development and progression of thymoma.

Gene Symbol :

NCBI Gene ID :

Uniprot Entry :

Construct Details :
The recombinant human VNN2-Fc fusion is expressed as a 697 amino acid protein consisting of Gln23 – Ser492 region of VNN2 (Uniprot Accession #O95498) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Source :
Human cells stably expressing VNN2-Fc growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :
10 20 30 40 50 60 QDSFIAAVYE HAVILPNKTE TPVSQEDALN LMNENIDILE TAIKQAAEQG ARIIVTPEDA 70 80 90 100 110 120 LYGWKFTRET VFPYLEDIPD PQVNWIPCQD PHRFGHTPVQ ARLSCLAKDN SIYVLANLGD 130 140 150 160 170 180 KKPCNSRDST CPPNGYFQYN TNVVYNTEGK LVARYHKYHL YSEPQFNVPE KPELVTFNTA 190 200 210 220 230 240 FGRFGIFTCF DIFFYDPGVT LVKDFHVDTI LFPTAWMNVL PLLTAIEFHS AWAMGMGVNL 250 260 270 280 290 300 LVANTHHVSL NMTGSGIYAP NGPKVYHYDM KTELGKLLLS EVDSHPLSSL AYPTAVNWNA 310 320 330 340 350 360 YATTIKPFPV QKNTFRGFIS RDGFNFTELF ENAGNLTVCQ KELCCHLSYR MLQKEENEVY 370 380 390 400 410 420 VLGAFTGLHG RRRREYWQVC TLLKCKTTNL TTCGRPVETA STRFEMFSLS GTFGTEYVFP 430 440 450 460 470 480 EVLLTEIHLS PGKFEVLKDG RLVNKNGSSG PILTVSLFGR WYTKDSLYSS TGTHTCPPCP 490 500 510 520 530 540 APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK 550 560 570 580 590 600 PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 610 620 630 640 650 660 LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL 670 680 690 TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK M.W.: Calculated molecular mass (kDa): 78.7; Estimated by SDS-PAGE under reducing condition (kDa): 80-85 Calculated PI: 6.34 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 107690 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: VNN2 hydrolyzes D-pantetheine and releases cysteamine. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Genomics 53:203 (1998) 2. J. Immunol. 162:4277 (1999) 3. Immunogenetics. 53:296 (2001) 4. Cancer Sci. 94:809 (2003) 5. J. Invest. Dermatol. 129: 2167 (2009) Documentation Product datasheet (pdf) can be downloaded here: FCL2117-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.

M.W. :
: 6.34

Calculated PI :

Calculated Extinction Coefficients :

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)

Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
VNN2 hydrolyzes D-pantetheine and releases cysteamine.

Molecule Class :
GPI-anchored Protein

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
VNN2; vanin 2; Vanin-2; FOAP-4; GPI-80

Research Area :

Pathway/Disease :
Cell Adhesion

Species :

CD Antigen :

References :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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