Name :
Human CEA/CEACAM5/CD66e Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
CEA (carcinoembryonic antigen), also kown as CEACAM5, BGP1 and CD66e, is a member of the CEA gene family belonging to the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPIanchored within two subgroups: the CEA cell adhesion molecules (CEACAM) and the pregnancy-specific glycoproteins. In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEA or CEACAM-5 contains1 Ig-like V-type (IgV) domain at the N-terminus, followed by 6 Ig-like C2-type (IgC2) domain and a GPI anchor. It exists as a homodimer . Intriguingly orthologs of human CEA have not been found in other species. Human CEA is expressed primarily in epithelial cells and functions as an adhesion molecule through homophilic and heterophilic interactions with CEACAM1/CD66a. CEA plays an important role in the development and maintenance of epithelial architecture. CEA is up-regulated in a wide array of human tumors and has been used a s a common cancer biomarker. CEA can promote tumor cell migration, invasion, adhesion, and metastasis. It may also contribute to tumorigenicity by inhibiting cellular differentiation. GPI-anchored CEA can be released by GPIPLD, resulting in a soluble molecule that induces tumor metastasis. CEA has been considered as a a target for immunotherapy.
Gene Symbol :
NCBI Gene ID :
1048
Uniprot Entry :
P06731
Construct Details :
The recombinant human CEA-Fc fusion is expressed as a 886 amino acid protein consisting of Lys35 – Gly682 region of CEA (Uniprot Accession #P06731) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing CEA-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 KLTIESTPFN VAEGKEVLLL VHNLPQHLFG YSWYKGERVD GNRQIIGYVI GTQQATPGPA 70 80 90 100 110 120 YSGREIIYPN ASLLIQNIIQ NDTGFYTLHV IKSDLVNEEA TGQFRVYPEL PKPSISSNNS 130 140 150 160 170 180 KPVEDKDAVA FTCEPETQDA TYLWWVNNQS LPVSPRLQLS NGNRTLTLFN VTRNDTASYK 190 200 210 220 230 240 CETQNPVSAR RSDSVILNVL YGPDAPTISP LNTSYRSGEN LNLSCHAASN PPAQYSWFVN 250 260 270 280 290 300 GTFQQSTQEL FIPNITVNNS GSYTCQAHNS DTGLNRTTVT TITVYAEPPK PFITSNNSNP 310 320 330 340 350 360 VEDEDAVALT CEPEIQNTTY LWWVNNQSLP VSPRLQLSND NRTLTLLSVT RNDVGPYECG 370 380 390 400 410 420 IQNKLSVDHS DPVILNVLYG PDDPTISPSY TYYRPGVNLS LSCHAASNPP AQYSWLIDGN 430 440 450 460 470 480 IQQHTQELFI SNITEKNSGL YTCQANNSAS GHSRTTVKTI TVSAELPKPS ISSNNSKPVE 490 500 510 520 530 540 DKDAVAFTCE PEAQNTTYLW WVNGQSLPVS PRLQLSNGNR TLTLFNVTRN DARAYVCGIQ 550 560 570 580 590 600 NSVSANRSDP VTLDVLYGPD TPIISPPDSS YLSGANLNLS CHSASNPSPQ YSWRINGIPQ 610 620 630 640 650 660 QHTQVLFIAK ITPNNNGTYA CFVSNLATGR NNSIVKSITV SASGTSPGST TENLYFQGST 670 680 690 700 710 720 GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG 730 740 750 760 770 780 VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG 790 800 810 820 830 840 QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD 850 860 870 880 GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK M.W.: Calculated molecular mass (kDa): 98; Estimated by SDS-PAGE under reducing condition (kDa): 80-90, 110-120, and 140-150. Calculated PI: 5.82 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 134745 Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure. Endotoxin Level: Biological Activity: Binds human Galectin-4 and CEACAM1/5 via a homophilic and heterophilic interaction. Blocks CEA-mediated cell-cell adhesion and cell migration. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Proc. Natl. Acad. Sci. 85:4648 (1988). 2. Mol. Cell. Biol. 10: 2738 (1990). 3. Cancer Res. 51:5679 (1991). 4. J. Exp. Med. 185:1557 (1997). 5. Semin. Cancer Biol. 9:67 (1999). 6. J. Biol. Chem. 275: 26935 (2000). Documentation Product datasheet (pdf) can be downloaded here: FCL2139B-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 5.82
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds human Galectin-4 and CEACAM1/5 via a homophilic and heterophilic interaction. Blocks CEA-mediated cell-cell adhesion and cell migration.
Molecule Class :
GPI-Anchored Protein
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CEA; CEACAM5; CEACAM-5; CD66e; CEAM5
Research Area :
Cancer
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
CD66e
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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