Name :
Human MSR1/SRA/SCARA1/phSR1/CD204 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
MSR1 (macrophage scavenger receptor 1), also known as CD204 and SCARA1 (scavenger receptor class A member 1), is a single-pass, type II membrane protein. It belongs to an expanding family of transmembrane molecules collectively referred to as scavenger receptors. Receptors of this family bind to a series of negativelycharged/polyanionic ligands. They recognize and internalize modified low density lipoproteins (LDLs) in the removal of many foreign substances and waste materials in the living body. Scavenger receptors can be categorized into 3 classes, and MSR1 is the class A receptors with 3 different types generated by alternative splicing. The extracellular region of MSR1 contains an αhelical coiledcoil domain, a collagenlike domain, and a cysteinerich domain (CRD) at the C-terminus. MSR1 is a macrophage-specific trimeric transmembrane glycoprotein involved in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer’s disease, and host defense. The isoforms type 1 (MSR-AI or SR-AI) and type 2 (MSR-AII or SR-AII) are functional receptors, which mediate the endocytosis of modified LDLs. The isoform type 3 (MSR-AIII or SR-AIII) does not internalize modified LDLs. It has an altered intracellular processing and is trapped within the endoplasmic reticulum. Thus the isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect. MSR1 may also play important roles in macrophage growth, cell adhesion, and osteoclast differentiation.
Gene Symbol :
NCBI Gene ID :
4481
Uniprot Entry :
P21757
Construct Details :
The recombinant human MSR1/CD204-Fc fusion is expressed as a 603 amino acid protein consisting of Lys77 – Leu451 region of MSR1/CD204 (Uniprot Accession #P21757) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing MSR1-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 KWETKNCSVS STNANDITQS LTGKGNDSEE EMRFQEVFME HMSNMEKRIQ HILDMEANLM 70 80 90 100 110 120 DTEHFQNFSM TTDQRFNDIL LQLSTLFSSV QGHGNAIDEI SKSLISLNTT LLDLQLNIEN 130 140 150 160 170 180 LNGKIQENTF KQQEEISKLE ERVYNVSAEI MAMKEEQVHL EQEIKGEVKV LNNITNDLRL 190 200 210 220 230 240 KDWEHSQTLR NITLIQGPPG PPGEKGDRGP TGESGPRGFP GPIGPPGLKG DRGAIGFPGS 250 260 270 280 290 300 RGLPGYAGRP GNSGPKGQKG EKGSGNTLTP FTKVRLVGGS GPHEGRVEIL HSGQWGTICD 310 320 330 340 350 360 DRWEVRVGQV VCRSLGYPGV QAVHKAAHFG QGTGPIWLNE VFCFGRESSI EECKIRQWGT 370 380 390 400 410 420 RACSHSEDAG VTCTLSTGTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV 430 440 450 460 470 480 DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS 490 500 510 520 530 540 NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS LTCLVKGFYP SDIAVEWESN 550 560 570 580 590 600 GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK M.W.: Calculated molecular mass (kDa): 66.9; Estimated by SDS-PAGE under reducing condition (kDa): 95-100 Calculated PI: 6.03 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 73630 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.25 mg/ml in sterile PBS pH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: Binds to advanced glycation endproducts of bovine serum albumin (AGEBSA) by ELISA FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Proc. Natl. Acad. Sci. 87: 9133 (1990). 2. J. Biol. Chem. 268:2120(1993). 3. J. Lipid Res. 34:983 (1993). 4. J. Clin. Invest. 108:649 (2001). 5. Cancer. Res. 63: 3486 (2003). 6. J. Immunol. 173: 6427 (2004). Documentation Product datasheet (pdf) can be downloaded here: FCL2292-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 6.03
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.25 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to advanced glycation endproducts of bovine serum albumin (AGEBSA) by ELISA
Molecule Class :
1-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
MSR1; SRA; SR-A; CD204; phSR1; phSR2; SCARA1
Research Area :
Metabolism
Pathway/Disease :
Macrophage Differentiation
Species :
Human
CD Antigen :
CD204
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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