Name :
Human CD116/GMR/CSF2RA/GMCSFR Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD116, also known as GM-CSFRα (granulocyte macrophage colony stimulating factor receptor alpha), is a component of the receptor complex that mediates cellular responses to GMCSF. This receptor complex is composed of a cytokine-specific alpha- chain CD116 and a beta-chain CD131 shared with the receptors for IL-3 and IL-5. CD116 alone binds GM-CSF with low affinity, whereas CD131 does not bind GM-CSF by itself. Association with CD131 converts CD116 from a low affinity to a high affinity receptor for GMCSF. GMCSF is a hematopoietic cytokine that regulates differentiation, survival, and proliferation of colony-forming unit-granulocyte-macrophage progenitor cells. It promotes the differentiation and mobilization of granulocytemacrophage, erythroid, megakaryocyte, and eosinophil progenitors. GMCSF also enhances the activation of myeloid cell effector functions and plays an important role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity. CD116 is a single pass, type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a 54 aa cytoplasmic tail that mediates the activation of Janus kinase (Jak)- Stat and MAPK as well as phosphatidylinositol 3-kinase (PI3K) pathways. CD116 is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, and placenta as well as in those nonhematopoietic tumor cells. Soluble forms of CD116 retain the ability to bind GMCSF.
Gene Symbol :
NCBI Gene ID :
1438
Uniprot Entry :
P15509
Construct Details :
The recombinant human CD116-Fc fusion is expressed as a 536 amino acid protein consisting of Asp23 – Gly320 region of CD116 (Uniprot Accession #P15509) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing CD116-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EKSDLRTVAP ASSLNVRFDS RTMNLSWDCQ ENTTFSKCFL TDKKNRVVEP RLSNNECSCT 70 80 90 100 110 120 FREICLHEGV TFEVHVNTSQ RGFQQKLLYP NSGREGTAAQ NFSCFIYNAD LMNCTWARGP 130 140 150 160 170 180 TAPRDVQYFL YIRNSKRRRE IRCPYYIQDS GTHVGCHLDN LSGLTSRNYF LVNGTSREIG 190 200 210 220 230 240 IQFFDSLLDT KKIERFNPPS NVTVRCNTTH CLVRWKQPRT YQKLSYLDFQ YQLDVHRKNT 250 260 270 280 290 300 QPGTENLLIN VSGDLENRYN FPSSEPRAKH SVKIRAADVR ILNWSSWSEA IEFGSDDGST 310 320 330 340 350 360 TENLYFQGST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP 370 380 390 400 410 420 EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP 430 440 450 460 470 480 IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY 490 500 510 520 530 KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK M.W.: Calculated molecular mass (kDa): 61.2; Estimated by SDS-PAGE under reducing condition (kDa): 75-80 Calculated PI: 8.45 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 81790 Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: Inhibits GMCSFdependent proliferation of human erythroleukemic TF-1 cells. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. EMBO J. 8:3667 (1989) 2. Proc. Natl. Acad. Sci. 87:9655 (1990) 3. Proc. Natl. Acad. Sci. 88: 5082 (1991) 4. J. Biol. Chem. 268:11881 (1993). 5. Crit. Rev. Immunol. 25:405 (2005) Documentation Product datasheet (pdf) can be downloaded here: FCL2268-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 8.45
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Inhibits GMCSFdependent proliferation of human erythroleukemic TF-1 cells.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD116; GMR; CSF2R; CSF2RA; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; GM-CSF-R-alpha; GMR-alpha
Research Area :
Immunology
Pathway/Disease :
Cytokine Signaling
Species :
Human
CD Antigen :
CD116
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
GABARAPL1/GEC-1 Protein
HSD17B14 Protein
Popular categories:
ADAM18
Signal Regulatory Protein gamma
