Name :
Human CD2 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
CD2, also known as T-cell surface antigen T11/Leu-5 and SRBC (sheep red blood cell receptor), is a single-pass type I transmembrane protein of the immunoglobulin superfamily (IgSF). CD2 contains one Ig-like C2-type motif and one Ig-like V-type motifs in the extracellular domain (ECD). CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells. CD2 is one of the earliest T-cell markers present on more than 95% of thymocytes. CD2 is also found on some natural killer (NK) cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or closely associated with it. CD2 interacts with lymphocyte function-associated antigen (CD58/LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells and its cytoplasmic domain is implicated in the signaling function by binding to intracellular proteins. CD2 may set quantitative thresholds in T cell activation both in vitro and in vivo. The complex network of CD2-CD58 signaling also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes.
Gene Symbol :
NCBI Gene ID :
914
Uniprot Entry :
P06729
Construct Details :
The recombinant human CD2-Fc is expressed as a 425-amino acid protein consisting of Lys25 – Asp209 region of CD2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing CD2-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 KEITNALETW GALGQDINLD IPSFQMSDDI DDIKWEKTSD KKKIAQFRKE KETFKEKDTY 70 80 90 100 110 120 KLFKNGTLKI KHLKTDDQDI YKVSIYDTKG KNVLEKIFDL KIQERVSKPK ISWTCINTTL 130 140 150 160 170 180 TCEVMNGTDP ELNLYQDGKH LKLSQRVITH KWTTSLSAKF KCTAGNKVSK ESSVEPVSCP 190 200 210 220 230 240 EKGLDGSSTT ENLYFQGSTG THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 250 260 270 280 290 300 VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK 310 320 330 340 350 360 VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE 370 380 390 400 410 420 SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK M.W.: Calculated molecular mass (kDa): 48.1; Estimated by SDS-PAGE under reducing condition (kDa): 60-65 Calculated PI: 8.18 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 65485 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure. Endotoxin Level: Biological Activity: Binds to human CD58/LFA3 protein by ELISA and blocks CD58/LFA3’s binding to cell surface CD2 and mediated signaling activity. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. J. Exp. Med. 166:923 (1987) 2. Nature 329:840 (1987) 3. J. Immunol. 160:2862 (1998) 4. Cell 97:791 (1999) 5. Proc. Natl. Acad. Sci. 96:4289 (1999) Documentation Product datasheet (pdf) can be downloaded here: FCL2552B-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 8.18
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to human CD58/LFA3 protein by ELISA and blocks CD58/LFA3’s binding to cell surface CD2 and mediated signaling activity.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD2; T11; SRBC; LFA-2; Leu-5
Research Area :
Immunology
Pathway/Disease :
T Cell Activation
Species :
Human
CD Antigen :
CD2
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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