Name :
Human CD172a/SIRPA/SHPS1/MYD1/PTPNS1 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD172a, also known as SIRPA (signal regulatory protein alpha), SHPS-1 (SHP substrate 1) and MyD-1 (Myeloid/Dendritic-1), is single pass type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. CD172a contains 1 Ig-like V-type and 2 Ig-like C1-type domains in the extracellular region and a cytoplasmic tail with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated. CD172a is involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes induced by cell adhesion, growth factors or insulin. CD172a is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It is also found on neurons, smooth muscle and endothelial cells. CD172a participates in signal transduction mediated by various growth factor receptors. CD172a supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. CD172a mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 has been demonstrated to be a ligand for CD172a. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. The CD47 integrin-CD172a interaction may promote macrophage fusion during osteoclastogenesis.
Gene Symbol :
NCBI Gene ID :
140885
Uniprot Entry :
P78324
Construct Details :
The recombinant human CD172a-Fc is expressed as a 568-amino acid protein consisting of Asp31 – Arg370 region of CD172a with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD172a-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EEELQVIQPD KSVLVAAGET ATLRCTATSL IPVGPIQWFR GAGPGRELIY NQKEGHFPRV 70 80 90 100 110 120 TTVSDLTKRN NMDFSIRIGN ITPADAGTYY CVKFRKGSPD DVEFKSGAGT ELSVRAKPSA 130 140 150 160 170 180 PVVSGPAARA TPQHTVSFTC ESHGFSPRDI TLKWFKNGNE LSDFQTNVDP VGESVSYSIH 190 200 210 220 230 240 STAKVVLTRE DVHSQVICEV AHVTLQGDPL RGTANLSETI RVPPTLEVTQ QPVRAENQVN 250 260 270 280 290 300 VTCQVRKFYP QRLQLTWLEN GNVSRTETAS TVTENKDGTY NWMSWLLVNV SAHRDDVKLT 310 320 330 340 350 360 CQVEHDGQPA VSKSHDLKVS AHPKEQGSNT AAENTGSNER VDKTHTCPPC PAPELLGGPS 370 380 390 400 410 420 VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST 430 440 450 460 470 480 YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT 490 500 510 520 530 540 KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ 550 560 GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass (kDa): 62.9 Estimated by SDS-PAGE under reducing condition (kDa): 80-90
Calculated PI :
6.53
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 72600
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand human CD47 protein and supports the adhesion of human red blood cells to the plate immobilized with CD172a protein
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD172A; SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; PTPNS1
Research Area :
Immunology
Pathway/Disease :
Macrophage Activation
Species :
Human
CD Antigen :
CD172a
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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