Name :
Human CTGF Protein, Fc-fusion, Biotinylated, Recombinant
Description :
Connective Tissue Growth Factor (CTGF) is a member of the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a multimodular organization. Each CCN member contains an IGFbinding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF binds multiple ligands and shows effects in development, differentiation, and tumorigenesis. CTGF is a mitogen that is secreted by vascular endothelial cells. It plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. The Cterminal cysteine knot motif contains the heparin and low-density lipoprotein receptor (LDLR) binding sites that contributes to the adhesive and mitogenic properties of CTGF. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. CTGF is the central mediator of tissue remodeling and fibrosis. It is implicated in tumor progression and metastasis. It promotes epithelial to mesenchymal transition (EMT), a process whereby normal epithelial cells become migratory, matrix-producing fibroblasts important for invasion and metastasis. Elevated CTGF levels are found in desmoplastic cancer where it supports the formation of the extensive fibrous connective tissue (desmoplasia) around cancer. Blockade of the expression or activity of CTGF reduces tumor growth and metastasis in serveral cancer animal model.
Gene Symbol :
NCBI Gene ID :
1490
Uniprot Entry :
Q5M8T4
Construct Details :
The recombinant human CTGF-Fc fusion is expressed as a 561 amino acid protein consisting of Gln27 – Ala349 region of CTGF2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions
Source :
Human cells stably expressing human CTGF-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QNCSGPCRCP DEPAPRCPAG VSLVLDGCGC CRVCAKQLGE LCTERDPCDP HKGLFCDFGS 70 80 90 100 110 120 PANRKIGVCT AKDGAPCIFG GTVYRSGESF QSSCKYQCTC LDGAVGCMPL CSMDVRLPSP 130 140 150 160 170 180 DCPFPRRVKL PGKCCEEWVC DEPKDQTVVG PALAAYRLED TFGPDPTMIR ANCLVQTTEW 190 200 210 220 230 240 SACSKTCGMG ISTRVTNDNA SCRLEKQSRL CMVRPCEADL EENIKKGKKC IRTPKISKPI 250 260 270 280 290 300 KFELSGCTSM KTYRAKFCGV CTDGRCCTPH RTTTLPVEFK CPDGEVMKKN MMFIKTCACH 310 320 330 340 350 360 YNCPGDNDIF ESLYYRKMYG DMASTTENLY FQGSTGTHTC PPCPAPELLG GPSVFLFPPK 370 380 390 400 410 420 PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL 430 440 450 460 470 480 TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT 490 500 510 520 530 540 CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS 550 560 VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 62.2 Estimated by SDS-PAGE under reducing condition (kDa): 65, 50 kDa (two distinct bands)
Calculated PI :
8.07
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 62570
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to anti-CTGF antibodies (SKU#MAB1466 & MAB1998) in a functional ELISA
Molecule Class :
Growth Factor (secreted)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CTGF; CCN2; NOV2; HCS24; IGFBP; IBP-8; IGFBP-8
Research Area :
Cancer
Pathway/Disease :
Tissue Remodeling
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CD40L/CD154/TRAP Protein
Cryptic Protein
Popular categories:
Interferon Gamma Inducible Protein 16
Carbonic Anhydrase 10
