Name :
Human LIGHT/TNFSF14/LTg/CD258/HVEML Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
LIGHT, also known as TNFSF14, HVEML and CD258, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. LIGHT is a single-pass type II transmebrane glycoprotein consisting of a 37 amino acid (aa) cytoplasmic domain, a 21-aa transmembrane domain (TM) and a 182 aa extracellular domain (ECD). LIGHT is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. LIGHT is known to bind at lest 2 TNF receptor superfamily (TNFRSF) members: HVEM, which is predominantly expressed by T cells, and LTβR (lymphotoxin β receptor), which is expressed by stromal cells and nonlymphoid hematopoietic cells. LIGHT also binds to a soluble TNFRSF member, DcR3 (decoy receptor 3), which can modulate the function of LIGHT. LIGHT functions as a co-stimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. LIGHT activates NF-kappaB, stimulates the proliferation of T cells, and triggers apoptosis of various tumor cells. LIGHT is also reported to prevent TNF-mediated apoptosis in primary hepatocyte. LIGHT has been shown to suppress tumor formation through the up-regulation of intercellular adhesion molecule 1 and the increase of lymphocyte adhesion to cancer cells. LIGHT is thus a promising target for cancer treatment.
Gene Symbol :
LIGHT; TNFSF14; LTg; CD258; HVEML
NCBI Gene ID :
8740
Uniprot Entry :
O43557
Construct Details :
The recombinant human LIGHT-Fc fusion is expressed as a 409 amino acid protein consisting of Gln60 – Val240 region of LIGHT and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing condition
Source :
Human cells stably expressing human LIGHT-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QLHWRLGEMV TRLPDGPAGS WEQLIQERRS HEVNPAAHLT GANSSLTGSG GPLLWETQLG 70 80 90 100 110 120 LAFLRGLSYH DGALVVTKAG YYYIYSKVQL GGVGCPLGLA STITHGLYKR TPRYPEELEL 130 140 150 160 170 180 LVSQQSPCGR ATSSSRVWWD SSFLGGVVHL EAGEKVVVRV LDERLVRLRD GTRSYFGAFM 190 200 210 220 230 240 VSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY 250 260 270 280 290 300 VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK 310 320 330 340 350 360 AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL 370 380 390 400 DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 45.5 Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI :
8.07
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 75330
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its receptors, HVEM and LTβR proteins, and induces receptor-mediated signaling activities including the stimulation of T cell proliferation and apoptosis in tumor cells
Molecule Class :
1-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
LIGHT; TNFSF14; LTg; CD258; HVEML
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD258
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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