Name :
Human CD32A/FCGR2A/FCGRIIa Protein, ECD (Extracellular Domain), Biotinylated, Recombinant
Description :
There are 3 classes of receptors for the Fc region of IgG (FcγRs): FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), each with multiple isoforms. All these receptors are members of the Ig superfamily (IgSF) and they play important roles in the activation or inhibition of immune responses. FcγRI is a high-affinity receptor for IgG while both FcγRII and FcγRIII are low-affinity receptors that bind IgG in the form of immune complexes. There are 3 genes for human FcγRII (CD32A, B, & C) that encodes single pass type I transmembrane proteins. The extracellular domain (ECD) of 3 human FcγRIIs shares ~90% amino acid sequence homology containing 2 Ig-like C2-type motifs. CD32A and CD32C contain immunoreceptor tyrosine-based activation motifs (ITAMs) while CD32B has an inhibitory motif (ITIM) in their cytoplasmic regions. Together they act as an activating or inhibiting receptor pair to regulate immune responses. CD32A is expressed on many immune cells including macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells. Signaling through CD32A results in the initiation of inflammatory responses such as cytolysis, phagocytosis, degranulation and cytokine production that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides Fc, CD32A also binds C-reactive protein (CRP). Two allelic variants (R167 and H167) of CD32A differ in their ability to bind human IgG2 or CRP.
Gene Symbol :
20 30 40 50 60
NCBI Gene ID :
2212
Uniprot Entry :
P12318
Construct Details :
The recombinant human CD32a ECD is expressed as a 205 amino acid protein consisting of Gln34 – Gly217 region of CD32a and a C-terminal His-tag. It contains 2 potential sites for N-linked glycosylation
Source :
Human cells stably expressing human CD32a ECD and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QAAAPPKAVL KLEPPWINVL QEDSVTLTCQ GARSPESDSI QWFHNGNLIP THTQPSYRFK 70 80 90 100 110 120 ANNNDSGEYT CQTGQTSLSD PVHLTVLSEW LVLQTPHLEF QEGETIMLRC HSWKDKPLVK 130 140 150 160 170 180 VTFFQNGKSQ KFSHLDPTFS IPQANHSHSG DYHCTGNIGY TLFSSKPVTI TVQVPSMGSS 190 200 SPMGSTTENL YFQGSTGHHH HHHHH
M.W. :
Calculated molecular mass (kDa): 23; Estimated by SDS-PAGE under reducing condition (kDa): 35-45
Calculated PI :
6.49
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 29700
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds human IgG or Fc-fusion proteins with low affinity as measured by a functional ELISA (estimated Kd of ~100 nM)
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD32A; FCGR2A; FCG2; FcGR; CD32; CDw32; FCGR2; IGFR2; FCGR2A1
Research Area :
Immunology
Pathway/Disease :
Fc-Binding
Species :
Human
CD Antigen :
CD32A
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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