Name :
Human OX40L/TNFSF4/GP34/CD252/TXGP1/CD134L Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
OX40L (OX40 ligand), also known as TNFSF4, gp34 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. OX40L is a single-pass type II transmebrane glycoprotein consisting of a 23 amino acid (aa) cytoplasmic domain, a 27-aa transmembrane domain (TM) and a 133 aa extracellular domain (ECD). OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40L exists as a homotrimer. OX40L binds to OX40/CD134, a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the regulation of T-cell-mediated immunity that includes B7, CD40L, CD30L, CD27L/CD70 and 4-1BBL. OX40L functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in the OX40L gene have been associated with Sjogren’s syndrome and systemic lupus erythematosus. The interaction of OX40L:OX40 is also involved in the pathogenesis of carotid artery disease and cancer. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.
Gene Symbol :
LIGHT; TNFSF14; LTg; CD258; HVEML
NCBI Gene ID :
7292
Uniprot Entry :
P23510
Construct Details :
The recombinant human OX40L-Fc fusion is expressed as a 361 amino acid protein consisting of Gln51 – Leu183 region of OX40L and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing condition.
Source :
Human cells stably expressing human OX40L-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QVSHRYPRIQ SIKVQFTEYK KEKGFILTSQ KEDEIMKVQN NSVIINCDGF YLISLKGYFS 70 80 90 100 110 120 QEVNISLHYQ KDEEPLFQLK KVRSVNSLMV ASLTYKDKVY LNVTTDNTSL DDFHVNGGEL 130 140 150 160 170 180 ILIHQNPGEF CVLSTGTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV 190 200 210 220 230 240 SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK 250 260 270 280 290 300 ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ 310 320 330 340 350 360 PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 41; Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI :
6.9
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 46340
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its receptor OX40 protein and induces OX40-mediated signaling activities
Molecule Class :
1-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
OX40L; TNFSF4; GP34; CD252; TXGP1; CD134L; OX-40L; TNLG2B
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD252
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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