Name :
Human CD39/ENTPD1/SPG64/NTPDase-1 Protein, ECD (Extracellular Domain), Recombinant
Description :
CD39, also known as ENTPD1 (ectonucleoside triphosphate diphosphohydrolase1) and NTPDase-1, is a two pass, type II transmembrane protein with an extracellular active site that hydrolyzes extracellular ATP and ADP to AMP. CD39 can hydrolyze ATP and ADP equally well. CD39 was originally discovered as a B lymphocyte cell surface marker, but it is also expressed on the surface of natural killer (NK) cells, T cells, neuron tissues and some endothelial cells. CD39 is involved in the processes of thromboregulation and vascular inflammation. In the central nervous system, CD39 hydrolyzes extracellular ATP and other nucleotides to regulate purinergic signaling and transmission. It is also implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. The mutations in the CD39 gene cause SPG64 (spastic paraplegia 64), an autosomal recessive neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. The administration of soluble ecto-enzyme of ENTPD1 may have therapeutic potential for treating some vascular and transplantationassociated diseases. Inhibition of this protein’s activity may confer anticancer benefits.
Gene Symbol :
NCBI Gene ID :
953
Uniprot Entry :
P49961
Construct Details :
The recombinant human CD39 ECD protein is expressed as a 452 amino acid protein consisting of Thr38- Val478 region of CD39 and a N-terminal His-tag. It contains 6 potential sites for N-linked glycosylation
Source :
Human cells stably expressing human CD39 ECD and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 MAHHHHHHHH STQNKALPEN VKYGIVLDAG SSHTSLYIYK WPAEKENDTG VVHQVEECRV 70 80 90 100 110 120 KGPGISKFVQ KVNEIGIYLT DCMERAREVI PRSQHQETPV YLGATAGMRL LRMESEELAD 130 140 150 160 170 180 RVLDVVERSL SNYPFDFQGA RIITGQEEGA YGWITINYLL GKFSQKTRWF SIVPYETNNQ 190 200 210 220 230 240 ETFGALDLGG ASTQVTFVPQ NQTIESPDNA LQFRLYGKDY NVYTHSFLCY GKDQALWQKL 250 260 270 280 290 300 AKDIQVASNE ILRDPCFHPG YKKVVNVSDL YKTPCTKRFE MTLPFQQFEI QGIGNYQQCH 310 320 330 340 350 360 QSILELFNTS YCPYSQCAFN GIFLPPLQGD FGAFSAFYFV MKFLNLTSEK VSQEKVTEMM 370 380 390 400 410 420 KKFCAQPWEE IKTSYAGVKE KYLSEYCFSG TYILSLLLQG YHFTADSWEH IHFIGKIQGS 430 440 450 DAGWTLGYML NLTNMIPAEQ PLSTPLSHST YV
M.W. :
Calculated molecular mass (kDa): 51.7; Estimated by SDS-PAGE under reducing condition (kDa): 65-70
Calculated PI :
6.14
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 77865
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.25 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Hydrolyzes the 5’-phosphate groups from the enzymatic substrate adenosine-5’-triphosphate (ATP)
Molecule Class :
2-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD39; ENTPD1; SPG64; ATPDase; NTPDase-1; Ecto-ATPase 1; Ecto-ATPDase 1
Research Area :
Immunology
Pathway/Disease :
Inflammatory Response
Species :
Human
CD Antigen :
CD39
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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