Name :
Human CD30L/CD153/TNFSF8 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD30L (CD30 ligand), also known as TNFSF8 and CD153, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily (TNFSF). CD30L/CD153 is a single-pass type II transmebrane glycoprotein consisting of a 37 amino acid (aa) cytoplasmic domain, a 25-aa transmembrane domain (TM) and a 172 aa extracellular domain (ECD). This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of CD30L expressed on B cell surface plays an inhibitory role in modulating Ig class switch. CD30L induces proliferation of T-cells. It enhances cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of CD30L on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin’s and some non-Hodgkin’s lymphomas.
Gene Symbol :
CD30L; CD153; TNFSF8; CD30-L; CD30LG; TNLG3A
NCBI Gene ID :
944
Uniprot Entry :
P32971
Construct Details :
The recombinant human CD30L-Fc fusion is expressed as a 400 amino acid protein consisting of Gln63 – Asp234 region of CD30L and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD30L-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QRTDSIPNSP DNVPLKGGNC SEDLLCILKR APFKKSWAYL QVAKHLNKTK LSWNKDGILH 70 80 90 100 110 120 GVRYQDGNLV IQFPGLYFII CQLQFLVQCP NNSVDLKLEL LINKHIKKQA LVTVCESGMQ 130 140 150 160 170 180 TKHVYQNLSQ FLLDYLQVNT TISVNVDTFQ YIDTSTFPLE NVLSIFLYSN SDSTGTHTCP 190 200 210 220 230 240 PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA 250 260 270 280 290 300 KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ 310 320 330 340 350 360 VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY 370 380 390 400 SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 45.2; Estimated by SDS-PAGE under reducing condition (kDa): 55-60
Calculated PI :
7.97
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 57465
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD30L; CD153; TNFSF8; CD30-L; CD30LG; TNLG3A
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD153
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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