Name :
Human CD40L/CD154/TNFSF5/gp39 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
CD40L (CD40 ligand), also known as TNFSF5 and CD154, is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily (TNFSF). CD40L/CD154 is a single-pass type II transmebrane glycoprotein consisting of a 22 amino acid (aa) cytoplasmic domain, a 24-aa transmembrane domain (TM) and a 215 aa extracellular domain (ECD). CD40L is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. CD40L co-stimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. A defect in the CD40L gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Gene Symbol :
CD40L; CD154; TNFSF5; IGM; IMD3; TRAP; gp39; HIGM1; T-BAM; hCD40L
NCBI Gene ID :
959
Uniprot Entry :
P29965
Construct Details :
The recombinant human CD40L-Fc fusion is expressed as a 443 amino acid protein consisting of His47 – Leu261 region of CD40L and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD40L-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 HRRLDKIEDE RNLHEDFVFM KTIQRCNTGE RSLSLLNCEE IKSQFEGFVK DIMLNKEETK 70 80 90 100 110 120 KENSFEMQKG DQNPQIAAHV ISEASSKTTS VLQWAEKGYY TMSNNLVTLE NGKQLTVKRQ 130 140 150 160 170 180 GLYYIYAQVT FCSNREASSQ APFIASLCLK SPGRFERILL RAANTHSSAK PCGQQSIHLG 190 200 210 220 230 240 GVFELQPGAS VFVNVTDPSQ VSHGTGFTSF GLLKLSTGTH TCPPCPAPEL LGGPSVFLFP 250 260 270 280 290 300 PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS 310 320 330 340 350 360 VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS 370 380 390 400 410 420 LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS 430 440 CSVMHEALHN HYTQKSLSLS PGK
M.W. :
Calculated molecular mass (kDa): 49.8; Estimated by SDS-PAGE under reducing condition (kDa): 65-70
Calculated PI :
7.97
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 48985
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD40L; CD154; TNFSF5; IGM; IMD3; TRAP; gp39; HIGM1; T-BAM; hCD40L
Research Area :
Immunology
Pathway/Disease :
B Cell Proliferation
Species :
Human
CD Antigen :
CD154
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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