Name :
Human CD14 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
CD14 is a GPI-anchored surface glycoprotein of the LRR (leucine-rich repeat) containing protein family. CD14 contains 11 LRR domains in the extracellular region. CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide (LPS). The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS. Thus CD14 belongs to the LPS receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial LPS. CD14 aslo acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface. CD14 also binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL.
Gene Symbol :
NCBI Gene ID :
929
Uniprot Entry :
P08571
Construct Details :
The recombinant human CD14-Fc fusion is expressed as a 553 amino acid protein consisting of Thr20 – Met345 region of CD14 and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD14-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP 70 80 90 100 110 120 RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA 130 140 150 160 170 180 TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL 190 200 210 220 230 240 DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN 250 260 270 280 290 300 SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP 310 320 330 340 350 360 EVDNLTLDGN PFLVPGTALP HEGSMVDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS 370 380 390 400 410 420 RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL 430 440 450 460 470 480 NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS LTCLVKGFYP 490 500 510 520 530 540 SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN 550 HYTQKSLSLS PGK
M.W. :
Calculated molecular mass (kDa): 60.7; Estimated by SDS-PAGE under reducing condition (kDa): 65-70
Calculated PI :
6.04
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 66765
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
GPI-anchored Protein
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD14
Research Area :
Immunology
Pathway/Disease :
LPS Signaling Pathway
Species :
Human
CD Antigen :
CD14
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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