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Name :
Human CD19 Protein, ECD (Extracellular Domain), Recombinant

Description :
CD19 is a single-pass type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily (IgSF). It contains two Ig-like C2-type domains. CD19 is an important pan B cell marker and co-stimulatory protein involved in growth regulation of B cells. CD19 is expressed only in B cells and follicular dendritic cells of the hematopoietic system. It is present on most pre-B cells and most non-T-cell acute lymphocytic leukemia cells (ALL) and B-cell type chronic lymphocytic leukemia cells (CLL). CD19 is a co-receptor for the antigen receptor complex on B cells to decrease the threshold for antigen receptor-dependent stimulation. It forms a complex with CD21, CD81 and CD225 in the membrane of B cells. It associates with GRB2 and SOS when activated and phosphorylated on Tyr-348 / Tyr-378. CD19 is a hallmark differentiation antigen of the B cell lineage and positively regulates antigen B-cell receptor (BCR) signal transduction for B cell selection, maturation, and differentiation. Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3), which is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of antibody while the numbers of circulating B cells is usually in the normal range, but can be low.

Gene Symbol :

NCBI Gene ID :

Uniprot Entry :

Construct Details :
The recombinant human CD19 ECD is expressed as a 271 amino acid protein consisting of Pro20 – Val279 region of CD19 (UniProt accession #P15391) and a C-terminal His-tag. It contains 5 potential sites for N-linked glycosylation.

Source :
Human cells stably expressing human CD19 ECD and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :

M.W. :
Calculated molecular mass (kDa): 29.9; Estimated by SDS-PAGE under reducing condition (kDa): ~45 probably due to glycosylation

Calculated PI :

Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 61355

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S” and “M” for marker)

Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
Binds anti-CD19 monoclonal antibody human IgG1 (SKU#0776) with high affinity (KD

Molecule Class :
1-Pass Type I Transmembrane

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
CD19; B4; CVID3; Leu-12

Research Area :

Pathway/Disease :
B Cell Development

Species :

CD Antigen :

References :
1. J. Exp. Med. 168:1205-1210 (1988) 2. J. Immunol. 143:712-717 (1989) 3. J Immunol. 158:4662-9 (1997). 4. Leuk Lymphoma. 43:613-6 (2002) 5. N. Engl. J. Med. 354:1901-1912 (2006)

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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