Name :
Human HVEM ECD (Extracellular Domain) Protein, Fc-fusion, ADCC-KO Mutant, Biotinylated, Recombinant
Description :
HVEM (herpesvirus entry mediator), also referred to as CD270, TR2 and ATAR, is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF14). HVEM is widely expressed, with the highest expression in lung, spleen and thymus. It is found in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain (ECD) of HVEM contains three cysteinerich repeats characteristic of TNF receptor superfamily members. The ECD of HVEM interacts directly with the herpes simplex virus (HSV) envelope glycoprotein D, contributing partly to HSV entry mechanism. The short cytoplasmic region of HVEM lacks a death domain, but contains a TRAF interaction motif for recruiting TRAFs and transducing signaling pathways that activate inflammatory and inhibitory T-cell immune responses. Two TNF superfamily ligands lymphotoxin-α (also known as TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM. Both ligands initiate the positive signaling activities. However, recent studies have also revealed that HVEM may be involved in the inhibitory pathway for T cells through serving as a receptor for BTLA (B and T lymphocyte attenuator) or CD272.
Gene Symbol :
TNFRSF14; CD270; TR2; ATAR; HVEA; LIGHTR; UNQ329/PRO509
NCBI Gene ID :
8764
Uniprot Entry :
Q92956
Construct Details :
The recombinant human HVEM-Fc fusion is expressed as a 388 amino acid protein consisting of Leu39 – Ser199 region of HVEM (UniProt accession #Q92956) and a C-terminal Fc (which is an ADCC-defective or knock-out (KO) mutant version containing E233P;L234V;L235A;ΔG236;A327G;A330S mutations) from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted).
Source :
Human cells stably expressing human HVEM-Fc-KO mutant and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 LPSCKEDEYP VGSECCPKCS PGYRVKEACG ELTGTVCEPC PPGTYIAHLN GLSKCLQCQM 70 80 90 100 110 120 CDPAMGLRAS RNCSRTENAV CGCSPGHFCI VQDGDHCAAC RAYATSSPGQ RVQKGGTESQ 130 140 150 160 170 180 DTLCQNCPPG TFSPNGTLEE CQHQTKCSWL VTKAGAGTSS STGTHTCPPC PAPELLGGPS 190 200 210 220 230 240 VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST 250 260 270 280 290 300 YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT 310 320 330 340 350 360 KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ 370 380 GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass (kDa): 42.4; Estimated by SDS-PAGE under reducing condition (kDa): 55-60
Calculated PI :
6.62
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 48495
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligands, BTLA/CD272 (SKU#FCL0722), TNFβ and LIGHT, and inhibits TNFβ- and LIGHTmediated signaling acitivities including cytotoxicity in L-929 mouse fibroblast cells.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TNFRSF14; CD270; TR2; ATAR; HVEA; LIGHTR; UNQ329/PRO509
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD270
References :
1. Cell 87:427 (1996). 2. J. Biol. Chem. 272:13471 (1997). 3. Immunity 8:21 (1998). 4. Immunol. Rev.202: 49 (2004). 5. Proc. Natl. Acad. Sci. 102: 13218 (2005).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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