Name :
Human LILRB2/CD85d/ILT4/LIR2/MIR10 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
LILRB2 (Leukocyte immunoglobulin like receptor B2), also known as CD85d, LIR2 (Leukocyte immunoglobulin-like receptor 2), ILT4 (Ig-like transcript 4), and MIR10 (myeloid inhibitory receptor 10), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF). It belongs to the subfamily B class of LIR receptors (LILRBs), which are single-pass type I trnasmembrane proteins with 2 or 4 extracellular Ig-like domains, a transmembrane domain (TM), and 2-4 cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB2 has 4 Ig-like C2 type domains and 3 TIIMs. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases to modulate cellular responses. LILRB2 is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. It is also detected at low levelson natural killer (NK) cells. Like LILRB1, LILRB2 binds to MHC class I molecules on antigen-presenting cells (APCs) and transduces a negative signal that inhibits stimulation of an immune response. LILRB2 may control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Engagement of LILRB2 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. LILRB2 also binds FCGR1A.
Gene Symbol :
NCBI Gene ID :
Uniprot Entry :
Q8N423
Construct Details :
The recombinant human LILRB2-Fc fusion is expressed as a 667 amino acid protein consisting of Gln22 – Val461 region of LILRB2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human LILRB2-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QTGTIPKPTL WAEPDSVITQ GSPVTLSCQG SLEAQEYRLY REKKSASWIT RIRPELVKNG 70 80 90 100 110 120 QFHIPSITWE HTGRYGCQYY SRARWSELSD PLVLVMTGAY PKPTLSAQPS PVVTSGGRVT 130 140 150 160 170 180 LQCESQVAFG GFILCKEGED EHPQCLNSQP HARGSSRAIF SVGPVSPNRR WSHRCYGYDL 190 200 210 220 230 240 NSPYVWSSPS DLLELLVPGV SKKPSLSVQP GPVMAPGESL TLQCVSDVGY DRFVLYKEGE 250 260 270 280 290 300 RDLRQLPGRQ PQAGLSQANF TLGPVSRSYG GQYRCYGAHN LSSECSAPSD PLDILITGQI 310 320 330 340 350 360 RGTPFISVQP GPTVASGENV TLLCQSWRQF HTFLLTKAGA ADAPLRLRSI HEYPKYQAEF 370 380 390 400 410 420 PMSPVTSAHA GTYRCYGSLN SDPYLLSHPS EPLELVVSGP SMGSSPPPTG PISTPAGPED 430 440 450 460 470 480 QPLTPTGSDP QSGLGRHLGV DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT 490 500 510 520 530 540 CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK 550 560 570 580 590 600 CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK NQVSLTCLVK GFYPSDIAVE 610 620 630 640 650 660 WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK
M.W. :
Calculated molecular mass (kDa): 73.3; Estimated by SDS-PAGE under reducing condition (kDa): 2 major bands 75-85 & 50-60 kDa probably due to proteolytic cleavage within the ECD of LILRB2
Calculated PI :
7.31
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 103220
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
LILRB2; ILT4; LIR2; MIR10; CD85d; ILT-4; LIR-2; MIR-10
Research Area :
Immunology
Pathway/Disease :
MHC-I Receptor Activity
Species :
Human
CD Antigen :
CD85d
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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