Name :
Human BST2/CD317/Tetherin Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD317, also known as BST-2 (bone marrow stromal antigen 2) and Tetherin, is a single pass type Ⅱ transmembrane protein with a unique topology. CD317 consists of an N-terminal cytoplasmic tail, followed by a transmembrane domain, an extracellular domain or loop, and a C-terminal GPI-linkage. The extracellular loop contains a coiled coil domain that forms a long semi-flexible rod-like structure, which may be important for virion retention at the cell surface and prevention of virus spreading. In addition the GPI anchor is essential for its antiviral activity. CD317 is expressed predominantly in liver, lung, heart and placenta. It is also expressed on bone marrow stromal cells and is upregulated in breast cancer, astrocytoma, and multiple myeloma cells. CD317 is highly expressed during B-cell development, from pro-B precursors to plasma cells, and on T-cells, monocytes, NK cells and dendritic cells. CD317 is involved in B-cell development and growth. CD317 is an interferon (IFN)-induced antiviral host restriction factor that efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. CD317 is active against at least 4 virus families: retroviruses, filoviruses, arenaviruses, and herpesviruses. CD317 can act as an inhibitor to HIV-1 infection in the absence of Vpu and inhibit the release of other viruses such as the Lassa, Marburg virions, and Kaposi sarcoma virus. CD317 binds to ILT7 on plasmacytoid dendritic cells and may provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection.
Gene Symbol :
NCBI Gene ID :
684
Uniprot Entry :
Q10589
Construct Details :
The recombinant human CD317-Fc is expressed as a 349-amino acid protein consisting of Ser50 – Ser161 region of CD317 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD317-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 SEACRDGLRA VMECRNVTHL LQQELTEAQK GFQDVEAQAA TCNHTVMALM ASLDAEKAQG 70 80 90 100 110 120 QKKVEELEGE ITTLNHKLQD ASAEVERLRR ENQVLSVRIA DKKYYPSSQD SSTTENLYFQ 130 140 150 160 170 180 GSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY 190 200 210 220 230 240 VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK 250 260 270 280 290 300 AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL 310 320 330 340 DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 39.1; Estimated by SDS-PAGE under reducing condition (kDa): ~55
Calculated PI :
5.97
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 40380
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand human ILT7 protein and blocks its signaling activity.
Molecule Class :
1-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
BST2; BST-2; Tetherin; CD317
Research Area :
Cancer
Pathway/Disease :
B Cell Activation
Species :
Human
CD Antigen :
CD317
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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