Name :
Human Tie2 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
Tie1 (Tyrosine kinase with Ig and EGF homology domains 1) and Tie2 comprise a receptor tyrosine kinase (RTK) subfamily with unique structural features: two immunoglobulinlike (Ig) domains flanking three epidermal growth factor (EGF)like domains and followed by three fibronectin type IIIlike repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Tie1 and Tie2 are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis, and hematopoiesis. Tie2 is a receptor for the angiopoietin (ANG) family: ANG1, ANG2, and ANG3 (mouse)/ANG4 (human). Tie1 may also act as an ANG receptor, possibly in complex with Tie2. Tie2 regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading as well as the reorganization of the actin cytoskeleton and maintenance of vascular quiescence. Tie2 is required for normal angiogenesis and heart development during embryogenesis, and for post-natal hematopoiesis. Tie2 may activate or inhibit angiogenesis, depending on the context. Tie2 may have anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Tie2 can be proteolytic processing leads to the shedding of the extracellular domain (soluble Tie2 or sTie2). Tie2 may play a role in a range of diseases with a vascular component, including neovascularization of tumors, psoriasis and inflammation. Mutations in the Tie2 gene are associated with dominantly inherited venous malformations (VMCM).
Gene Symbol :
NCBI Gene ID :
7010
Uniprot Entry :
Q02763
Construct Details :
The recombinant human Tie2-Fc fusion is expressed as a 952 amino acid protein consisting of Ala23- Met746 region of Tie2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted).
Source :
Human cells stably expressing human Tie2-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
AMDLILINSLPLVSDAETSLTCIASGWRPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAK KVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKV LIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIV RRCEAQKWGPECNHLCTACMNNGVCHEDTGECICPPGFMGRTCEKACELHTFGRTCKERCSG QEGCKSYVFCLPDPYGCSCATGWKGLQCNEACHPGFYGPDCKLRCSCNNGEMCDRFQGCLCS PGWQGLQCEREGIPRMTPKIVDLPDHIEVNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLH PKDFNHTDHFSVAIFTIHRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPKPLNAPNVIDT GHNFAVINISSEPYFGDGPIKSKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTEYELCVQ LVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVER RSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQP ENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEP ETAYQVDIFAENNIGSSNPAFSHELVTLPESQAPADLGGGKMSTGTHTCPPCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYR VVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQV SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSC SVMHEALHNHYTQKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 106.3; Estimated by SDS-PAGE under reducing condition (kDa): 110-120
Calculated PI :
6.53
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 135855
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT:+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Recombinant Tie2 protein binds human Angiopoietin2 and inhibits Angiopoietin2 mediated signaling activity.
Molecule Class :
Receptor Tyrosine Kinase (RTK)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD202b; TEK; p140 TEK; VMCM; TIE-2; hTIE2; VMCM1
Research Area :
Cancer
Pathway/Disease :
Angiopoietin Signaling Pathway
Species :
Human
CD Antigen :
CD202b
References :
1. Cell 87:1181 (1996). 2. Science 277:55 (1997). 3. Immunity 9:677 (1998). 4. J. Biol. Chem. 274:30196 (1999). 5. Nat. Rev. Cancer 10:575 (2010)
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
IL-2R alpha/CD25 Protein
Carbonic Anhydrase 9 Protein
Popular categories:
B7-1/CD80
4-1BB/CD137
