Name :
Human BCAM/CD239 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD239, also known as BCAM (basal cell adhesion molecule) and Lu (Lutheran blood group glycoprotein), is a single pass type I transmembrane proteins of the Ig superfamily (IgSF). There are two alternatively spliced variants of CD239, which similar structures except for the cytoplasmic tails (one lacking a 40 amino acid SH3 motif). A polymorphism at position 77 within the extracellular domain (ECD) is the basis for the difference between the Lua and Lub Lutheran blood groups. CD239 contains 5 Ig-like (2 V-type and 3 C2-type) motifs in the extracellular domain (ECD). CD239 is widely expressed in epithelial and endothelial tissues. It is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle. CD239 shares a significant sequence similarity with the CD146/MUC18 and CD166/ALCAM. CD239 cooperates with Integrins β1 and αVβ3 as an adhesion receptor for Laminins. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. CD239 is upregulated on carcinomas (particularly ovarian), sarcomas, astrocytomas, and melanomas. In addition CD239 interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group.
Gene Symbol :
NCBI Gene ID :
4059
Uniprot Entry :
P50895
Construct Details :
The recombinant human CD239-Fc is expressed as a 754-amino acid protein consisting of Glu32 – Ala547 region of CD239 (UniProt accession #P50895) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD329-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EVRLSVPPLV EVMRGKSVIL DCTPTGTHDH YMLEWFLTDR SGARPRLASA EMQGSELQVT 70 80 90 100 110 120 MHDTRGRSPP YQLDSQGRLV LAEAQVGDER DYVCVVRAGA AGTAEATARL NVFAKPEATE 130 140 150 160 170 180 VSPNKGTLSV MEDSAQEIAT CNSRNGNPAP KITWYRNGQR LEVPVEMNPE GYMTSRTVRE 190 200 210 220 230 240 ASGLLSLTST LYLRLRKDDR DASFHCAAHY SLPEGRHGRL DSPTFHLTLH YPTEHVQFWV 250 260 270 280 290 300 GSPSTPAGWV REGDTVQLLC RGDGSPSPEY TLFRLQDEQE EVLNVNLEGN LTLEGVTRGQ 310 320 330 340 350 360 SGTYGCRVED YDAADDVQLS KTLELRVAYL DPLELSEGKV LSLPLNSSAV VNCSVHGLPT 370 380 390 400 410 420 PALRWTKDST PLGDGPMLSL SSITFDSNGT YVCEASLPTV PVLSRTQNFT LLVQGSPELK 430 440 450 460 470 480 TAEIEPKADG SWREGDEVTL ICSARGHPDP KLSWSQLGGS PAEPIPGRQG WVSSSLTLKV 490 500 510 520 530 540 TSALSRDGIS CEASNPHGNK RHVFHFGTVS PQTSQASTTE NLYFQGSTGT HTCPPCPAPE 550 560 570 580 590 600 LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE 610 620 630 640 650 660 EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP 670 680 690 700 710 720 SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD 730 740 750 KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK
M.W. :
Calculated molecular mass (kDa): 82.9; Estimated by SDS-PAGE under reducing condition (kDa): 100-110
Calculated PI :
5.67
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 101270
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Supports the adhesion of human cells such as osteogenic sarcoma cells to the surface immobilized with CD239-Fc protein.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
BCAM; AU; LU; MSK19; CD239
Research Area :
Cancer
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
CD239
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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