Name :
Human CD44 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD44 is a single-pass type I transmembrane glycoprotein in the cartilage link protein family. CD44 exists in multiple spliced forms with enormous variability in glycosylation. CD44 can range from 80 to 200 kDa in size. In the extracellular domain, CD44 has a hyaluronanbinding disulfidestabilized link region and a stem region with 10 variably spliced exons (v1 10, exons 6 15). The standard or hematopoietic form, CDw44, does not contain the variable segments. CD44 binds a variety of ligands in cell-cell and cell-matrix interactions and signal transduction. CD44 is the major receptor for hyaluronan to regulate cell migration, tumor growth and progression. CD44 is involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by pro-inflammatory agents. CD44 acts as a “platform” for growth factors and metalloproteinases. CD44 can also function as a coreceptor that modulates the activity of receptors such as c-MET and EGFR family of tyrosine kinases. CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form or can be cleaved at multiple sites by matrix metalloproteinases (MMPs). These cleavage events may promote metastasis by enhancing tumor cell motility and growth. Abnormal expression or dysfunction of CD44 causes numerous pathogenic phenotypes. The expression of specific CD44 isoforms correlates with cancer aggressiveness and T cell activation.
Gene Symbol :
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; PGP-I; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
NCBI Gene ID :
960
Uniprot Entry :
P16070
Construct Details :
The recombinant human CD44-Fc is expressed as a 470 amino acid protein consisting of Gln21 – Thr263 region of CDw44 (UniProt accession #P16070 – isoform 12 or CDw44) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image inserted).
Source :
Human cells stably expressing human CD44-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGF IEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGP ITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHP IPDEDSPWITDSTDRIPATRDQDTFHPSGGSHTTHGSESDGHSHGSQEGGANTTSGPIRTD KTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGV EVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP REPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSF FLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 52; Estimated by SDS-PAGE under reducing condition (kDa): 80-90
Calculated PI :
5.52
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 56560
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Immobilized CD44-Fc protein binds hyaluronan and anti-CD44 monoclonal antibodies, human IgG1 (SKU#MAB0503), mouse IgG2a (SKU#MAB0494) and rabbit IgG (SKU#MAB0492) with high affinity (KD
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; PGP-I; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Research Area :
Cancer
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
CD44
References :
1. Proc. Natl. Acad. Sci. 89:12160 (1992). 2. J. Biol. Chem. 271:20603 (1996). 3. Oncogene 22:1511 (2003). 4. Nat. Rev. Immunol. 4:931 (2004). 5. Cancer Res. 64:876 (2004).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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