Name :
Human Fc (IgG1) Control Protein, Biotinylated, Recombinant
Description :
The human IGHG1 (immunoglobulin heavy constant gamma 1 (G1m marker)) gene encodes the Ig gamma-1 (IgG1) chain constant (C) region. The C region contains 3 Ig-like domains including the CH1, hinge, CH2 and CH3 domains. The hinge, CH2 and CH3 region is also commonly referred to as the Fc region. Fc meditates the interactions with the complement components and Fc-gamma receptors (FCGRs) to induce innate immune responses such as antibody-dependent complement activation and phagocytosis, respectively. Diseases associated with IGHG1 include heavy chain deposition disease, and B-cell chronic lymphocytic leukemia (B-CLL). In addition a chromosomal translocation t(11;14)(q13;q32) within the IGHG1 locus is found in multiple myeloma (MM). MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. MM is is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia (PCL). The human Fc control protein is expressed as a recombinant protein that include the hinge , CH2 and CH3 domains of human IgG1 (which are identical to the Fc used in other Fc-fusion proteins). The purified protein can be used as a negative control for other Fc-fusion proteins in a variety of biological assays.
Gene Symbol :
IGHG1
NCBI Gene ID :
3500
Uniprot Entry :
P01857
Construct Details :
The recombinant human Fc control protein is expressed as a 227 amino acid protein consisting of Asp104 – Lys330 region of human IGHG1 (UniProt accession #P018571), which exists as a dimer under non-reducing condition (see the gel image inserted).
Source :
Human cells stably expressing human Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD 70 80 90 100 110 120 GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK 130 140 150 160 170 180 GQPREPQVYT LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS 190 200 210 220 DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK
M.W. :
Calculated molecular mass 25.6 kDa; estimated by SDS-PAGE under reducing condition 30-35 kDa probably due to glycosylation
Calculated PI :
7.18
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
Ig (secreted)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
IgGH1
Research Area :
Immunology
Pathway/Disease :
FCGR Signaling Pathway
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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