Name :
Human CD28H Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD28H (CD28 homolog), also known as TMIGD2 (transmembrane and immunoglobulin domain-containing protein 2) and IGPR1 (immunoglobulin and proline-rich receptor 1), is a single-pass type I transmembrane glycoprotein. CD28H contains 1 Ig (immunoglobulin)-like domain in the extracellular region and 1 cytoplasmic proline-rich domain. CD28H is widely expressed, mainly by epithelial and endothelial cells. It is also expressed in lymphoid organs, with highest levels in thymus, spleen, peripheral blood lymphocytes and liver. In the thymus, it is expressed in CD4 and CD8 single- and double-positive cells, but not in immature CD4 and CD8 double-negative cells. In peripheral blood mononuclear cells, CD28H is highly expressed on natural killer (NK) cells and CD3+ T-cells but not on B-cells. CD28H may form homophilic interactions that could regulate cell-cell interaction, cell migration, and angiogenesis. The B7 family member B7-H7 (B7 homolog 7), also know n as HHLA2 (human endogenous retrovirus-H long terminal repeat-associating protein 2), was identified as a specific ligand for CD28H. Through interaction with B7-H7, CD28H may co-stimulate T-cells in the context of TCR-mediated activation. The B7-H7/CD28H interaction selectively co-stimulates human T-cell growth and cytokine production via an AKT-dependent signalling cascade.
Gene Symbol :
CD28H; TMIGD2; IGPR1; IGPR-1;UNQ3059/PRO9879
NCBI Gene ID :
126259
Uniprot Entry :
Q96BF3
Construct Details :
The recombinant human CD28H-Fc fusion is expressed as a 366-amino acid protein consisting of Leu23 – Gly150 region of CD28H (UniProt accession #Q96BF3) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.
Source :
Human cells stably expressing CD28H-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 LSVQQGPNLL QVRQGSQATL VCQVDQATAW ERLRVKWTKD GAILCQPYIT NGSLSLGVCG 70 80 90 100 110 120 PQGRLSWQAP SHLTLQLDPV SLNHSGAYVC WAAVEIPELE EAEGNITRLF VDPDDPTQNR 130 140 150 160 170 180 NRIASFPGST TENLYFQGST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC 190 200 210 220 230 240 VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC 250 260 270 280 290 300 KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW 310 320 330 340 350 360 ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK
M.W. :
Calculated molecular mass 40.7 kDa; estimated by SDS-PAGE under reducing condition 55-60 kDa probably due to glycosylation
Calculated PI :
6.17
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free)
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its B7 family ligand, B7-H7/HHLA2 (SKU#: FCL1038) and iInhibit B7-H7-mediated signaling activity including IL-2 secretion in human T cells stimulated with anti-CD3 antibody.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD28H; TMIGD2; IGPR1; IGPR-1;UNQ3059/PRO9879
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD28H
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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