Name :
Human B7-H3b/4Ig-B7-H3 Protein, ECD, Fc-fusion, Biotinylated, Recombinant
Description :
B7H3 (B7 homolog 3), also known as CD276, is a single pass type I transmembrane glycoprotein that belongs to the B7 family of the Ig superfamily. Like other B7 family members, B7-H3 contains one Ig V-like and one Ig C-like domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. B7-H3 serves as an accessory modulator of T-cell response. It has been reported that B7-H3 can provide both a stimulatory and inhibitory signal to T cells. As a negative regulator, it preferentially affects Th1 responses. B7-H3 may also play an important role in muscle-immune interactions. A longer form of B7-H3 has been identified in human but not in mouse. It is termed 4IgB7H3 or B7H3b with two additional Iglike domains (one V-type and one C-type). B7-H3b appears to be the dominantly expressed form in human and is found on human dendritic cells, activated T, B and NK cells. Human B7H3b binding to an undefined receptor may inhibit NK cell killing and cytokine release. It is also involved in late stage osteoblast differentiation. B7-H3 is detected in a variety of cancer types and its expression in some cancers correlates with poor outcome of cancer patients.
Gene Symbol :
The recombinant human B7-H3b-Fc fusion is expressed as a 666 amino acid protein consisting of Leu29 – Ala466 region of B7-H3b or 4IgB7-H3 (UniProt accession #Q5ZPR3 – isoform 1) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition
NCBI Gene ID :
80381
Uniprot Entry :
Q5ZPR3
Construct Details :
The recombinant human B7-H3b-Fc fusion is expressed as a 666 amino acid protein consisting of Leu29 – Ala466 region of B7-H3b or 4IgB7-H3 (UniProt accession #Q5ZPR3 – isoform 1) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition
Source :
Human cells stably expressing B7-H3b-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 LEVQVPEDPV VALVGTDATL CCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FAEGQDQGSA 70 80 90 100 110 120 YANRTALFPD LLAQGNASLR LQRVRVADEG SFTCFVSIRD FGSAAVSLQV AAPYSKPSMT 130 140 150 160 170 180 LEPNKDLRPG DTVTITCSSY QGYPEAEVFW QDGQGVPLTG NVTTSQMANE QGLFDVHSIL 190 200 210 220 230 240 RVVLGANGTY SCLVRNPVLQ QDAHSSVTIT PQRSPTGAVE VQVPEDPVVA LVGTDATLRC 250 260 270 280 290 300 SFSPEPGFSL AQLNLIWQLT DTKQLVHSFT EGRDQGSAYA NRTALFPDLL AQGNASLRLQ 310 320 330 340 350 360 RVRVADEGSF TCFVSIRDFG SAAVSLQVAA PYSKPSMTLE PNKDLRPGDT VTITCSSYRG 370 380 390 400 410 420 YPEAEVFWQD GQGVPLTGNV TTSQMANEQG LFDVHSVLRV VLGANGTYSC LVRNPVLQQD 430 440 450 460 470 480 AHGSVTITGQ PMTFPPEAST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC 490 500 510 520 530 540 VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC 550 560 570 580 590 600 KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW 610 620 630 640 650 660 ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK
M.W. :
Calculated molecular mass 72.6 kDa; estimated by SDS-PAGE under reducing condition ~95 kDa and ~65 kDa probably representing the 4Ig-B7-H3 and 2Ig-B7-H3 isoforms
Calculated PI :
5.29
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition with two major products: 25% of 95 kDa and 75% of 65 kDa (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Inhibits antiCD3induced proliferation of stimulated human T cells. Binds anti-B7-H3 monoclonal antibodies, human IgG1 (SKU#MAB1755) and rabbit IgG (SKU#MAB1753) in a functional ELISA (see Technical Data)
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD276; B7H3; B7RP-2; 4Ig-B7-H3; PSEC0249; UNQ309/PRO352
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD276
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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