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Name :
Human 4-1BB/CD137 Protein, ECD, Fc-fusion, Biotinylated, Recombinant

Description :
4-­1BB, also known as CD137, ILA and TNFRSF9, is a single-pass type I transmembrane glycoprotein of the TNF receptor superfamily (TNFRSF). 4­1BB is expressed as a disulfide­linked homodimer on activated T cells. 4-1BB is absent from naive T cells, but its expression is induced by lymphocyte activation. 4-1BB is the receptor for 4-1BB ligand/TNFSF9, which is expressed on antigen-presenting cells such as macrophages and activated B cells. 4-1BB mediated signaling pathway contributes to the proliferation, survival, and development of T cells. 4-1BB can also promote proliferation in peripheral monocytes, enhance T cell apoptosis triggered by TCR-CD3 activation, and regulate CD28 co-stimulation to promote Th1 cell responses. 4­1BB deficient mice show augmented T cell activation. 4-1BB and its ligand are also expressed in different human tumor tissues. 4­-1BB activation by crosslinking with its ligand or agonistic antibody enhances cytotoxic T cell and NK cell mediated anti­tumor immunity. 4­-1BB can interact with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation. 4-1BB may also be involved in the development of inflammation in high fat diet­induced metabolic syndrome. The soluble form of 4­-1BB circulates at elevated levels in the serum of rheumatoid arthritis.

Gene Symbol :
4-1BB;CD137; ILA; TNFRSF9; CDw137; MGC2172

NCBI Gene ID :

Uniprot Entry :

Construct Details :
The recombinant human 4-1BB-Fc fusion is expressed as a 390 amino acid protein consisting of Leu24 – Gln186 region of 4-1BB (UniProt accession #Q07011) and a C-terminal Fc from human IgG1, which exists as a dimer/tetramer under non-reducing conditions (see the gel image above).

Source :
Human cells stably expressing human 4-1BB/CD137 and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :

M.W. :
Calculated molecular mass (kDa): 42.8; Estimated by SDS-PAGE under reducing condition (kDa): 55-60

Calculated PI :

Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 42535

Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)

Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
Binds to its ligand human 4-1BBL and anti-4-1BB monoclonal antibodies (SKU#MAB1829) with high affinity by ELISA (see Technical Data). Blocks 4-1BBL/4-1BB-­induced signaling activity.

Molecule Class :
1-Pass Type I Transmembrane

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
CD137; ILA; TNFRSF9; CDw137; MGC2172

Research Area :

Pathway/Disease :
T Cell Costimulation

Species :

CD Antigen :

References :
1. J. Immunol. 168:4897 (2002). 2. Nat. Immunol. 9:917 (2008). 3. Trends Pharmacol. Sci. 29(8): 383 (2008). 4. Immunol. Rev. 229:192 (2009). 5. Diabetes 60:3159 (2011).

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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