Share this post on:

Name :
Human ICOS Protein, ECD, Fc-fusion, biotinylated, recombinant

Description :
CD278, also known as ICOS (inducible co-stimulator), is a single-pass type I transmembrane glycoprotein that belongs to the CD28/CTLA4 (cytotoxic T lymphocyte-associated antigen 4, or CD152) family of the immunoglobulin (Ig) superfamily. Like other family members, CD28, CTLA4 and PD-1, CD278/ICOS contains one Ig-like V-type domain in the extracellular region and shares 39% amino acid similarity with CD28 and CTLA­4. ICOS is expressed on most CD45RO+ cells and is up­regulated after the activation on Th primed cells. B7­H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand (ICOSL). Optimal T cell activation and expansion are regulated by signals delivered not only through the T cell receptor (TCR), but also a number of co-stimulatory molecules, including ICOS and ICOSL. ICOS is expressed on antigen-primed T cells, and ICOSL is expressed mainly on B cells and macrophages. The interaction of B7-H2/ICOS plays critical roles in T cell dependent B cell activation and in Th cell differentiation. These T−B cell interactions are essential for germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. In addition, ICOS also regulates TH2-mediated mucosal inflammatory responses, and aberrant expression of ICOS has been implicated in allergic inflammatory responses and other immune disorders.

Gene Symbol :

NCBI Gene ID :

Uniprot Entry :

Construct Details :
The recombinant human ICOS-Fc fusion protein is expressed as a 348-amino acid protein consisting of Glu21 – Lys140 region of ICOS (UniProt accession #Q9Y6W8) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Source :
Human cells stably expressing ICOS-Fc and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :

M.W. :
Calculated molecular mass 39.2 kDa; estimated by SDS-PAGE under reducing condition ~55 kDa probably due to glycosylation

Calculated PI :

Calculated Extinction Coefficients :

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)

Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
Binds to its ligand ICOSL/B7-H2/CD275 and inhibits human T cell proliferation induced by ICOSL in the presence of anti-CD3e antibody.

Molecule Class :
1-Pass Type I Transmembrane

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :

Research Area :

Pathway/Disease :
T Cell Costimulation

Species :

CD Antigen :

References :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites:
Popular product recommendations:
IFN-gamma Protein
PCSK9 Protein
Popular categories:

Share this post on: