Name :
Human LFA3/AG3/CD58 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
LFA3 (lymphocyte function-associated antigen 3), also known as Ag3 and CD58, is single-pass type I transmembrane or GPI-anchored glycoprotein belonging to the immunoglobulin superfamily (IgSF). LFA3 contains 1 Ig-like C2-type domain in its extracellular region. LFA3 is primarily expressed on Antigen Presenting Cells (APCs), particularly macrophages. LFA3 is the ligand of the T-lymphocyte CD2 glycoprotein and functions in adhesion and activation of T lymphocytes. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and antigen-dependent interactions of T-lymphocytes with target cells. It is important in strengthening the adhesion between the T cells and Professional APCs. This adhesion occurs as part of the transitory initial encounters between T cells and APCs before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes that the T-cell receptors are reactive to. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA3+ cells and is important for the T-lymphocyte rosetting with erythrocytes. Polymorphisms in the LFA3 gene are associated with increased risk for multiple sclerosis.
Gene Symbol :
NCBI Gene ID :
965
Uniprot Entry :
P19256
Construct Details :
The recombinant human LFA3-Fc is expressed as a 319-amino acid protein consisting of Phe29 – Val120 region of LFA3 with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image above).
Source :
Human cells stably expressing human LFA3-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 FSQQIYGVVY GNVTFHVPSN VPLKEVLWKK QKDKVAELEN SEFRAFSSFK NRVYLDTVSG 70 80 90 100 110 120 SLTIYNLTSS DEDEYEMESP NITDTMKFFL YVDKTHTCPP CPAPELLGGP SVFLFPPKPK 130 140 150 160 170 180 DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV 190 200 210 220 230 240 LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL 250 260 270 280 290 300 VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM 310 HEALHNHYTQ KSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 36.2 Estimated by SDS-PAGE under reducing condition (kDa): 50-55.
Calculated PI :
6.11
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 50225
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its receptor human CD2 (SKU#FCL2552) and increases T cell proliferation and cytokine secretion in the presence of anti-CD3 antibody.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD58; ag3; LFA3; LFA-3
Research Area :
Immunology
Pathway/Disease :
T Cell Activation
Species :
Human
CD Antigen :
CD58
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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