Name :
Human CD114/CSF3R/GCSFR Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD114, also known as CSF3R and GCSFR (Granulocyte Colony Stimulating Factor Receptor), is a cell surface receptor for CSF3 (Colony Stimulating Factor 3) that plays a critical role in the proliferation, differentiation, and activation of hematopoietic cells of the neutrophil lineage. CD114 is a member of the cytokine receptor superfamily and the immunoglobulin (Ig) superfamily that may also function in some other cell surface adhesion or recognition processes. CD114 contains an immunoglobulin (Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region (ECD). CD114 is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of its ligand induces dimerization or oligomerization of this receptor and leads to the activation of several intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase (Lyn and Syk) pathways. CD114 thus plays important functional roles in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the GCSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood.
Gene Symbol :
NCBI Gene ID :
1441
Uniprot Entry :
Q99062
Construct Details :
The recombinant human CD114-Fc fusion is expressed as a 839 amino acid protein consisting of Glu25 – Glu625 region of CD114 (Uniprot Accession #Q99062) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing CD114-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 ECGHISVSAP IVHLGDPITA SCIIKQNCSH LDPEPQILWR LGAELQPGGR QQRLSDGTQE 70 80 90 100 110 120 SIITLPHLNH TQAFLSCCLN WGNSLQILDQ VELRAGYPPA IPHNLSCLMN LTTSSLICQW 130 140 150 160 170 180 EPGPETHLPT SFTLKSFKSR GNCQTQGDSI LDCVPKDGQS HCCIPRKHLL LYQNMGIWVQ 190 200 210 220 230 240 AENALGTSMS PQLCLDPMDV VKLEPPMLRT MDPSPEAAPP QAGCLQLCWE PWQPGLHINQ 250 260 270 280 290 300 KCELRHKPQR GEASWALVGP LPLEALQYEL CGLLPATAYT LQIRCIRWPL PGHWSDWSPS 310 320 330 340 350 360 LELRTTERAP TVRLDTWWRQ RQLDPRTVQL FWKPVPLEED SGRIQGYVVS WRPSGQAGAI 370 380 390 400 410 420 LPLCNTTELS CTFHLPSEAQ EVALVAYNSA GTSRPTPVVF SESRGPALTR LHAMARDPHS 430 440 450 460 470 480 LWVGWEPPNP WPQGYVIEWG LGPPSASNSN KTWRMEQNGR ATGFLLKENI RPFQLYEIIV 490 500 510 520 530 540 TPLYQDTMGP SQHVYAYSQE MAPSHAPELH LKHIGKTWAQ LEWVPEPPEL GKSPLTHYTI 550 560 570 580 590 600 FWTNAQNQSF SAILNASSRG FVLHGLEPAS LYHIHLMAAS QAGATNSTVL TLMTLTPEGS 610 620 630 640 650 660 ESTTENLYFQ GSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH 670 680 690 700 710 720 EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL 730 740 750 760 770 780 PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE 790 800 810 820 830 NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK M.W.: Calculated molecular mass (kDa): 93.7; Estimated by SDS-PAGE under reducing condition (kDa): 100-110 Calculated PI: 6.22 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 178770 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: Binds CSF3/G-CSF and inhibits CSF3/GCSFinduced proliferation of NFS-60 myelogenous leukemia lymphoblast cells with a typical ED50 of 0.5-3 μg/ml FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. J. Exp. Med. 172:1559 (1989) 2. EMBO J. 10:2855 (1990) 3. Proc. Natl. Acad. Sci. 91:4480 (1993) 4. J. Exp. Med. 190:497 (1998) 5. Front. Biosci. 12:800 (2006) Documentation Product datasheet (pdf) can be downloaded here: FCL2197-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 6.22
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds CSF3/G-CSF and inhibits CSF3/GCSFinduced proliferation of NFS-60 myelogenous leukemia lymphoblast cells with a typical ED50 of 0.5-3 μg/ml
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD114/CSF3R/GCSFR Gene
Research Area :
Hematology
Pathway/Disease :
Amelogenesis
Species :
Human
CD Antigen :
CD114
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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