Name :
Human CD73/NT5E/NTE/5’-NT Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD73, also known as 5’-NT, NT5 and NTE, is a glycosyl-phosphatidyl-inositol (GPI) anchored cell membrane protein that belongs to the 5′-nucleotidase (5’-NT) family. The native enzyme exists as a homodimer on the cell surface. CD73 is a purine salvage enzyme expressed on human T and B lymphocytes. CD73 converts extracellular nucleoside5’monophosphates to membrane permeable nucleosides, with AMP as the preferred substrate. It also exhibits AMP-, NAD-, and NMN-nucleosidase activities. CD73 is one of several ectoenzymes responsible for the production of extracellular adenosine, a signaling molecule involved in many physiologic processes, including inflammation, tissue injury response, and cholinergic synapses. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors (GPCR) to induce intracellular responses. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5’nucleotidase activity. CD73 mediates lymphocyte-stromal cell interactions to facilitate lymphocyte development/function. CD73 has also been considered as a lymphocyte maturation marker independent of its catalytic activity. CD73 serves as a co-stimulatory molecule in activating T cells. The mutation in CD73 gene is associated with calcification of joints and arteries (CALJA), a condition characterized by adult-onset calcification of the lower extremity arteries and hand and foot capsule joints.
Gene Symbol :
NCBI Gene ID :
4907
Uniprot Entry :
P21589
Construct Details :
The recombinant human CD73-Fc fusion is expressed as a 759 amino acid protein consisting of Trp27 – Lys547 region of CD73 (Uniprot Accession #P21589) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing CD73-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ 70 80 90 100 110 120 YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA 130 140 150 160 170 180 KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK 190 200 210 220 230 240 LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI 250 260 270 280 290 300 VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK 310 320 330 340 350 360 WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHA DETFWNHVSM 370 380 390 400 410 420 CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST 430 440 450 460 470 480 GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD 490 500 510 520 530 540 GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI KSTTENLYFQ GSTGTHTCPP 550 560 570 580 590 600 CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK 610 620 630 640 650 660 TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV 670 680 690 700 710 720 YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS 730 740 750 KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK M.W.: Calculated molecular mass (kDa): 84.4; Estimated by SDS-PAGE under reducing condition (kDa): 85-90 Calculated PI: 6.41 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 93585 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: CD73 hydrolyzes the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP) with a specific activity over 20,000 pmoles/min/μg. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Cell Signal. 9: 131 (1997) 2. Immunol. Rev. 161:95 (1998). 3. Eur J Immunol. 28: 2981 (1998) 4. J. Biol. Chem. 278:13468 (2003) 5. J Immunol. 180: 4246 (2008) Documentation Product datasheet (pdf) can be downloaded here: FCL2199-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 6.41
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
CD73 hydrolyzes the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP) with a specific activity over 20,000 pmoles/min/μg.
Molecule Class :
GPI-anchored Protein
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD73; 5’-NT; NT; eN; NT5; NTE; eNT; E5NT; CALJA
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD73
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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