Name :
Human CD132/IL2RG/P64/CIDX/IMD4/SCIDX Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
CD132, also known as IL2RG (interleukin 2 receptor subunit gamma), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily. CD132 consists of an extracellular region with 1 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif. The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD132 is a common component of the receptor complexeas for a variety of interleukins, including IL2, IL4, IL7, IL15, IL21 and probably IL13. CD132 is thus referred to as the common gamma chain. Mutations in the CD132 gene cause severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
Gene Symbol :
NCBI Gene ID :
3561
Uniprot Entry :
P31785
Construct Details :
The recombinant human CD132/IL2RG-Fc fusion is expressed as a 468 amino acid protein consisting of Leu23 – Ala262 region of CD132/IL2RG and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD132/IL2RG-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 LNTTILTPNG NEDTTADFFL TTMPTDSLSV STLPLPEVQC FVFNVEYMNC TWNSSSEPQP 70 80 90 100 110 120 TNLTLHYWYK NSDNDKVQKC SHYLFSEEIT SGCQLQKKEI HLYQTFVVQL QDPREPRRQA 130 140 150 160 170 180 TQMLKLQNLV IPWAPENLTL HKLSESQLEL NWNNRFLNHC LEHLVQYRTD WDHSWTEQSV 190 200 210 220 230 240 DYRHKFSLPS VDGQKRYTFR VRSRFNPLCG SAQHWSEWSH PIHWGSNTSK ENPFLFALEA 250 260 270 280 290 300 STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV 310 320 330 340 350 360 DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA 370 380 390 400 410 420 KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD 430 440 450 460 SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass (kDa): 53.8; Estimated by SDS-PAGE under reducing condition (kDa): 75-80
Calculated PI :
6.33
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 97580
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
IL2RG; CD132; P64; CIDX; IMD4; SCIDX; IL-2RG; SCIDX1; XCID; XSCID
Research Area :
Immunology
Pathway/Disease :
Cytokine Signaling
Species :
Human
CD Antigen :
CD132
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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