Name :
Human CD13/ANPEP/APN/LAP1 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD13, also known as ANPEP (alanyl aminopeptidase), APM (aminopeptidase M) and APN (aminopeptidase N), is a single pass type II transmembrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a 935 amino acid (aa) extracellular domain (ECD). The large ECD contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. CD13 is a broad specificity aminopeptidase belonging to the Peptidase M1 family. CD13 may be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. CD13 located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. CD13 cleaves antigen peptides bound to major histocompatibility complex class II molecules of antigen presenting cells and to degrade neurotransmitters at synaptic junctions. CD13 Is also implicated as a regulator of IL-8 bioavailability in the endometrium, contributing to angiogenesis. CD13 is a receptor for human coronavirus that is a cause of upper respiratory tract infections. It also mediates human cytomegalovirus (HCMV) infection. Defects in the CD13 gene may be a cause of various types of leukemia or lymphoma. CD13 plays a critical role in the pathogenesis of cholesterol gallstone disease.
Gene Symbol :
NCBI Gene ID :
290
Uniprot Entry :
P15144
Construct Details :
The recombinant human CD13-Fc protein is expressed as a 1161 amino acid protein consisting of Gln34 – Lys767 region of CD13 fused to the Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD13-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QEKNKNANSS PVASTTPSAS ATTNPASATT LDQSKAWNRY RLPNTLKPDS YRVTLRPYLT 70 80 90 100 110 120 PNDRGLYVFK GSSTVRFTCK EATDVIIIHS KKLNYTLSQG HRVVLRGVGG SQPPDIDKTE 130 140 150 160 170 180 LVEPTEYLVV HLKGSLVKDS QYEMDSEFEG ELADDLAGFY RSEYMEGNVR KVVATTQMQA 190 200 210 220 230 240 ADARKSFPCF DEPAMKAEFN ITLIHPKDLT ALSNMLPKGP STPLPEDPNW NVTEFHTTPK 250 260 270 280 290 300 MSTYLLAFIV SEFDYVEKQA SNGVLIRIWA RPSAIAAGHG DYALNVTGPI LNFFAGHYDT 310 320 330 340 350 360 PYPLPKSDQI GLPDFNAGAM ENWGLVTYRE NSLLFDPLSS SSSNKERVVT VIAHELAHQW 370 380 390 400 410 420 FGNLVTIEWW NDLWLNEGFA SYVEYLGADY AEPTWNLKDL MVLNDVYRVM AVDALASSHP 430 440 450 460 470 480 LSTPASEINT PAQISELFDA ISYSKGASVL RMLSSFLSED VFKQGLASYL HTFAYQNTIY 490 500 510 520 530 540 LNLWDHLQEA VNNRSIQLPT TVRDIMNRWT LQMGFPVITV DTSTGTLSQE HFLLDPDSNV 550 560 570 580 590 600 TRPSEFNYVW IVPITSIRDG RQQQDYWLID VRAQNDLFST SGNEWVLLNL NVTGYYRVNY 610 620 630 640 650 660 DEENWRKIQT QLQRDHSAIP VINRAQIIND AFNLASAHKV PVTLALNNTL FLIEERQYMP 670 680 690 700 710 720 WEAALSSLSY FKLMFDRSEV YGPMKNYLKK QVTPLFIHFR NNTNNWREIP ENLMDQYSEV 730 740 750 760 770 780 NAISTACSNG VPECEEMVSG LFKQWMENPN NNPIHPNLRS TVYCNAIAQG GEEEWDFAWE 790 800 810 820 830 840 QFRNATLVNE ADKLRAALAC SKELWILNRY LSYTLNPDLI RKQDATSTII SITNNVIGQG 850 860 870 880 890 900 LVWDFVQSNW KKLFNDYGGG SFSFSNLIQA VTRRFSTEYE LQQLEQFKKD NEETGFGSGT 910 920 930 940 950 960 RALEQALEKT KANIKWVKEN KEVVLQWFTE NSKSTGTHTC PPCPAPELLG GPSVFLFPPK 970 980 990 1000 1010 1020 PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL 1030 1040 1050 1060 1070 1080 TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT 1090 1100 1110 1120 1130 1140 CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS 1150 1160 VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 131.6; Estimated by SDS-PAGE under reducing condition (kDa): 180-190
Calculated PI :
5.51
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 230280
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD13; ANPEP; APN; LAP1; P150; PEPN; GP150
Research Area :
Immunology
Pathway/Disease :
Neutrophil Degranulation
Species :
Human
CD Antigen :
CD13
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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